Dissecting Functional Correlates of a Double Mutation Enhancing GltPh Transport Efficiency using Alchemical Free Energy Calculations
نویسندگان
چکیده
منابع مشابه
Protein thermostability calculations using alchemical free energy simulations.
Thermal stability of proteins is crucial for both biotechnological and therapeutic applications. Rational protein engineering therefore frequently aims at increasing thermal stability by introducing stabilizing mutations. The accurate prediction of the thermodynamic consequences caused by mutations, however, is highly challenging as thermal stability changes are caused by alterations in the fre...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2016
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2015.11.3360